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INVESTIGATION OF METABOLISM OF SOME HUMAN ERYTHROCYTE PHOSPHATES USING 31 P-NMR UPON CRYOPRESERVATION WITH 1,2-PROPANEDIOL.

[In Russian. / En russe.]

Author(s) : ZINCENKO V. D.

Type of article: Article

Summary

THE KINETICS OF BREAKDOWN OF 2,3-DIPHOSPHOGLYCERATE (2,3-DPG) AND ACCUMULATION OF INORGANIC PHOSPHATE (P) HAS BEEN STUDIED IN HUMAN ERYTHROCYTES DURING INCUBATION WITHOUT GLUCOSE AT 310 K (37 DEG C) AFTER DIFFERENT STEPS OF CRYOPRESERVATION WITH 1,2-PROPANEDIOL. THE CONTENT OF 2,3-DPG WAS SHOWN TO INCREASE AFTER FREEZING TO 77 K (-196 DEG C) AND THAWING, AND TO DECREASE DURING INCUBATION WITHOUT GLUCOSE AT 310 K FASTER THAN IN INTACT CELLS. REMOVAL OF CRYOPROTECTANTS INCREASED THE RATE OF GROWING THE CONCENTRATION OF P AND DECREASING THE LEVEL OF 2,3-DPG.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1988-0390
  • Languages: Russian
  • Source: Kriobiologija - n. 2
  • Publication date: 1987
  • Document available for consultation in the library of the IIR headquarters only.

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