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Leakage of a trapped fluorescent marker from liposomes: effects of eutectic crystallization of sodium chloride and internal freezing.

Author(s) : KRISTIANSEN J.

Type of article: Article

Summary

Leakage was low after freezing between -15 and -35 deg C, but increased steeply between -35 and -45 deg C. From differential scanning calorimetry measurements it was found that the increase in leakage was associated with two crystallization processes: eutectic crystallization of sodium chloride and freezing of undercooled solvent trapped in the interior of the liposomes ("internal freezing"). Damage caused by the former process could effectively be prevented by small amounts of trehalose. A time-dependent process was found to cause leakage from the liposomes at -25 deg C. The association between leakage and thermal activity suggests that differential scanning calorimetry supplements cryomicroscopy and leakage measurements in the characterization of cryostability of liposomes.

Details

  • Original title: Leakage of a trapped fluorescent marker from liposomes: effects of eutectic crystallization of sodium chloride and internal freezing.
  • Record ID : 1994-1892
  • Languages: English
  • Source: Cryobiology - vol. 29 - n. 5
  • Publication date: 1992/10

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