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Morphology study of freeze-drying mononuclear cells of human cord blood.

Author(s) : LI J., HUA T. C., GU X. L., et al.

Type of article: Article

Summary

Research on haematopoietic stem cells of human cord blood is becoming more important. At present, cord blood is mainly preserved at ultra-low temperatures. In the former study, we compared the effects of preserving mononuclear cells (MNC) and whole human cord blood by freeze-drying. This study was conducted on freeze-drying mononuclear cells. Samples in the presence of PVP, sucrose, mannitol and FBS were frozen to -38 °C. Afterwards, they were vacuum-dried at a selected shelf temperature of -30 °C for the main drying stage, and then vacuum-dried at 15 °C for the second drying stage. The entire freeze-drying process took 41 hours. Samples were stored at room temperature for 7 days prior to evaluation. Subsequently, the dried samples were resuspended in an isotonic phosphate-buffered saline solution. The residual moisture content was 6.5 plus or minus 0.87%. Cell recovery was tested using a haemacytometer, and the numerical cell count recovery of rehydrated MNC increased by 8%. Morphology of the fresh and rehydrated MNC was analysed using standard light microscopy, scanning electron microscope and transmission electron microscope. The results showed that karyons changed and cytoplasm decreased after rehydration, but it is still unknown that whether these changes will influence the proliferative ability of the stem cells.

Details

  • Original title: Morphology study of freeze-drying mononuclear cells of human cord blood.
  • Record ID : 2006-0978
  • Languages: English
  • Source: CryoLetters - vol. 26 - n. 3
  • Publication date: 2005/05

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