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Freeze-drying of human platelets: influence of intracellular trehalose and extracellular protectants.

Author(s) : ZHOU X. L., ZHU H., ZHANG S. Z., et al.

Type of article: Article

Summary

The long-term preservation of human platelets in blood banks is difficult. The authors investigated freeze-drying. They used intracellular trehalose as lyoprotectant. Bovine serum albumin and trehalose were used as extracellular protectants. The results demonstrated that 13 mM intracellular trehalose exerted a beneficial effect on freeze-dried human platelets, and slighting improved the protection provided by extracellular protectants. The survival rate of rehydrated platelets was roughly 85%, the morphology was not affected and the aggregation percentage with thrombin (1 U/ml) was 97.3%.

Details

  • Original title: Freeze-drying of human platelets: influence of intracellular trehalose and extracellular protectants.
  • Record ID : 2006-2687
  • Languages: English
  • Source: CryoLetters - vol. 27 - n. 1
  • Publication date: 2006/01

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