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New thermal stress test to assess the viability of cryopreserved boar sperm.

Author(s) : FISER P. S., et al.

Type of article: Article

Summary

A new, rapid, thermal stress test for assessing the viability of boar semen, requiring only 45 min of incubation at 42.5 deg C, was developed and compared with a widely used stress test of 180 min incubation at 37 deg C. Results of both procedures were almost similar. Neither test was able to show differences in the kinetic rating of motile sperm after freezing in relation to the glycerol concentration present during freezing. The new test has also other advantages over the longer procedure, as far as concentrations of glycerol (up to 6%) are concerned, in relation with evaluation of damages of cells and quality of sperm. The new thermal stress test thus has the advantages of greater sensitivity and more rapid execution.

Details

  • Original title: New thermal stress test to assess the viability of cryopreserved boar sperm.
  • Record ID : 1992-3068
  • Languages: English
  • Source: Cryobiology - vol. 28 - n. 5
  • Publication date: 1991/10

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