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THE EFFECT OF GLYCEROL-RELATED OSMOTIC CHANGES ON POST-THAW MOTILITY AND ACROSOMAL INTEGRITY OF RAM SPERMATOZOA.

Author(s) : FISER P. S., FAIRFULL R. W.

Type of article: Article

Summary

RAM SEMEN, COLLECTED BY ARTIFICIAL VAGINA, WAS DILUTED AND PROCESSED FORLONG-TERM STORAGE. THE CONCENTRATION OF THE CRYOPROTECTANT, GLYCEROL, WAS AJUSTED TO 4% IN THE DILUTED SEMEN PRIOR TO FREEZING BY A ONE-STEP ADDITION AT 303 K (30 DEG C) (METHOD 1), BY COOLING THE SEMEN TO 278 K (5 DEG C) AND ADDITION OF THE GLYCEROL GRADUALLY OVER 30 MIN (METHOD 2), BY ONE-STEP ADDITION OF GLYCEROL PRIOR TO EQUILIBRATION FOR 2 HOURS (METHOD 3), OR BY COOLING TO 278 K, FOLLOWED BY A HOLDING PERIOD OF 2 HOURS AT 278 K, AND THE ONE-STEP ADDITION OF GLYCEROL JUST PRIOR TO FREEZING (METHOD 4). THESE DATA INDICATE THAT POST-THAW SURVIVAL OF SPERMATOZOA CAN BE INFLUENCED BY THE WAY IN WHICH GLYCEROL IS ADDED PRIOR TO FREEZING. HOWEVER, POST-THAW SPERMATOZOA MOTILITY AND ACROSOMAL INTEGRITY CAN BE MAINTAINED EVEN AFTER A RAPID DECREASE IN GLYCEROL CONCENTRATION SUCH AS THAT WHICH ACCOMPANIES INSEMINATION OR DILUTION OF SEMEN FOR ASSESSMENT OF MOTILITY.

Details

  • Original title: THE EFFECT OF GLYCEROL-RELATED OSMOTIC CHANGES ON POST-THAW MOTILITY AND ACROSOMAL INTEGRITY OF RAM SPERMATOZOA.
  • Record ID : 1990-0853
  • Languages: English
  • Source: Cryobiology - vol. 26 - n. 1
  • Publication date: 1989

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