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Development of a shoot-tip vitrification protocol and comparison with encapsulation-based procedures for plum (Prunus domestica L.) cryopreservation.

Author(s) : DE CARLO A., BENELLI C., LAMBARDI M.

Type of article: Article

Summary

Cryopreservation of plum (Prunus domestica L.), cv Regina Claudia, was obtained by a vitrification/one-step cooling procedure of shoot tips from cold-hardened in vitro-grown plants. Best survival (57%) was obtained when the shoot tips were precultured at 4 °C for 2 days on 0.09 M sucrose-containing Quoirin and Le Poivre medium, loaded for 30 min with a cryoprotectant (2 M glycerol and 0.4 M sucrose), incubated with the PVS2 solution at 0 °C for 90 min, and directly plunged into liquid nitrogen. Among the other cryogenic procedures tested, the performance of the encapsulation-vitrification method was similar to the vitrification protocol in terms of shoot-tip regrowth (47.5%), while encapsulation-dehydration was unsatisfactory.

Details

  • Original title: Development of a shoot-tip vitrification protocol and comparison with encapsulation-based procedures for plum (Prunus domestica L.) cryopreservation.
  • Record ID : 2001-2516
  • Languages: English
  • Source: CryoLetters - vol. 21 - n. 4
  • Publication date: 2000/07
  • Document available for consultation in the library of the IIR headquarters only.

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