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Optimal conditions of simple cold storage solution: essential and non-essential components in University of Wisconsin solution.

[In Japanese. / En japonais.]

Author(s) : SUMIMOTO R., DOI Y.

Type of article: Article

Summary

University of Wisconsin solution is an isotonic intracellular-type solution in which the chloride anion has been replaced by the larger molecular-weight anion lactobionate and the trisaccharide raffinose is used instead of glucose or mannitol. In addition, the solution contains a stable colloid, hydroxyethyl starch, phosphate buffer, magnesium sulfate, insulin, allopurinol and reduced glutathione with adenosine. Essential factors in UW and the possibility of the development of a new preservation solution are discussed. It is the use of lactobionate as a major anion in the UW solution that is responsible for its dramatic effectiveness. The solution has a high viscosity and a low buffering capacity. These limiting factors undermine ideal long-term preservation. By ameliorating these points it may be possible to develop a better preservation solution than standard UW solution.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 1996-1882
  • Languages: Japanese
  • Source: Low Temperature Medicine - vol. 21 - n. 1
  • Publication date: 1995/07
  • Document available for consultation in the library of the IIR headquarters only.

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