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Osmometric behaviour, hydraulic conductivity, and incidence of intracellular ice formation in bovine oocytes at different developmental stages.

Author(s) : RUFFING N. A., STEPONKUS P. L., PITT R. E., PARKS J. E.

Type of article: Article

Summary

Bovine oocytes that were immature, matured in vitro or in vivo, or matured and fertilized in vitro were studied using a microscope diffusion chamber to estimate osmotic parameters and a cryomicroscope to characterize intracellular ice formation. Linear Boyle-Van't Hoff relationships were observed with all four types of oocytes between 0.265 and 0.799 osm sodium chloride. At 20 deg C, estimates of hydraulic conductivity were significantly higher for matured in vitro oocytes than immature and in vivo oocytes. Other results are given.

Details

  • Original title: Osmometric behaviour, hydraulic conductivity, and incidence of intracellular ice formation in bovine oocytes at different developmental stages.
  • Record ID : 1994-2555
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 6
  • Publication date: 1993/12

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