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Osmotic characteristics of isolated human and canine pancreatic islets.

Author(s) : WOODS E. J., ZIEGER M. A. J., LAKEY J. R. T., LIU J., CRITSER J. K.

Type of article: Article

Summary

Cryopreservation protocols for pancreatic islets may be optimized by modeling the changes in volume that occur during cryoprotectant addition/dilution and cooling/warming. Modeling may be facilitated if it can be demonstrated that isolated islets behave as ideal osmometers where the equilibrium volume is a linear function of the reciprocal of the osmolality of the extracellular medium (the Boyle van't Hoff relationship). The present study was performed to test the hypotheses that: human and canine islets exhibit a linear osmotic response during exposure to hypo-, iso-, and hyperosmotic solutions and to determine the human and canine islets osmotically inactive fraction; to determine if the ionic properties of osmotic response of pancreatic islets varies between species (human and canine).

Details

  • Original title: Osmotic characteristics of isolated human and canine pancreatic islets.
  • Record ID : 1998-3192
  • Languages: English
  • Source: Cryobiology - vol. 35 - n. 2
  • Publication date: 1997/09

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