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OSMOTIC INJURY TO CELLS DURING FREEZING PRESERVATION.

Author(s) : GAO D. Y., KORNBLATT J. A., LIN S.

Summary

USING THE HUMAN ERYTHROCYTE AS A RESEARCH MODEL AND PCMBS (P-(CHLOROMERCURI) BENZENESULFONIC ACID) AS AN INHIBITOR OF WATER PERMEATION THROUGH THE CELL MEMBRANE, CRYOINJURIES OF CELLS TREATED OR NOT WITH PCMBS WERE EXPERIMETALLY EVALUATED TO INVESTIGATE THE SIGNIFICANCE OF OSMOTIC INJURIES AND MEANS OF AVOIDING THEM. GLYCEROL WAS USED AS A CRYOPROTECTIVE AGENT, AND THE GLYCEROL CONCENTRATION IN CELL SUSPENSION WAS VARIED. OSMOTIC INJURY OF HUMAN ERYTHROCYTES DURING THE FREEZING-THAWING PROCESS WAS VERY DETRIMENTAL TO THE SURVIVAL OF THE CELLS AND HENCE IT SHOULD BE PREVENTED ; AND GLYCEROL GREATLY PROTECTS CELLS AGAINST OSMOTIC INJURY. THE HIGHER THE INITIAL GLYCEROL CONCENTRATION, THE LESSER THE EXTENT OF OSMOTIC INJURY.

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Details

  • Original title: OSMOTIC INJURY TO CELLS DURING FREEZING PRESERVATION.
  • Record ID : 1992-2483
  • Languages: English
  • Source: New challenges in refrigeration. Proceedings of the XVIIIth International Congress of Refrigeration, August 10-17, 1991, Montreal, Quebec, Canada.
  • Publication date: 1991/08/10
  • Document available for consultation in the library of the IIR headquarters only.

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