
IIR document
16 YEAR CRYOGENIC STORAGE OF BLOOD FROZEN BY DROPLET FREEZING TECHNIQUE AND A LOW GLYCEROL-RAPID FREEZE PROCEDURE.
Author(s) : ROWE A. W.
Summary
CRYOPRESERVATION PROCEDURES FOR CELLS SUCH AS ERYTHROCYTES ARE OF LIMITED VALUE UNLESS THE TEMPERATURE OF STORAGE IS SUFFICIENTLY LOW. US REGULATIONS LIMIT STORAGE TO 3 YEARS, BUT DATA INDICATE THAT NO OUTDATING IS NECESSARY IF THE CELLS ARE STORED PROPERLY. TWO TECHNIQUES FOR RED CELL FREEZING WERE USED: (A) DROPLET FREEZING OF SMALL ALIQUOTS, PERFORMED BY SPRAYING RED CELLS SUSPENDED IN A SUGAR SOLUTION ONTO A MOVING FILM OF LIQUID NITROGEN OR (B) GLYCEROL FREEZING OF LARGE VOLUMES (500 MILLILITRES), USING THE LOW GLYCEROL-RAPID FREEZE PROCEDURE. AT 253 K (-20 DEG C), GROSS HEMOLYSIS WAS EVIDENT AT 24 HR AFTER THAWING. AT 193 K (-80 DEG C),DROPLET-FROZEN CELLS REMAINED STABLE FOR ABOUT 6 MONTHS, BUT GLYCEROL-FROZEN CELLS SHOWED EVIDENCE OF HEMOLYSIS WITHIN 1 WEEK. THESE DATA SUGGEST ICE CRYSTAL GROWTH WHICH CAUSES CELL DAMAGE AT 193 K. AT 108 AND 77 K (-165 AND -196 DEG C), DROPLET-FROZEN CELLS AND GLYCEROL-FROZEN CELLS REMAINED STABLE FOR OVER 16 YEARS.
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Details
- Original title: 16 YEAR CRYOGENIC STORAGE OF BLOOD FROZEN BY DROPLET FREEZING TECHNIQUE AND A LOW GLYCEROL-RAPID FREEZE PROCEDURE.
- Record ID : 1988-1681
- Languages: English
- Source: Development in refrigeration, refrigeration for development. Proceedings of the XVIIth international Congress of Refrigeration.
- Publication date: 1987/08/24
- Document available for consultation in the library of the IIR headquarters only.
Links
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Indexing
-
COMPARISON OF THE CRYOPROTECTION OF RED BLOOD C...
- Author(s) : BOUTRON P., ARNAUD F.
- Date : 1984
- Languages : English
- Source: Cryobiology - vol. 21 - n. 3
View record
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THE EFFECT OF COOLING RATE AND WARMING RATE ON ...
- Author(s) : PEGG D. E.
- Date : 1984
- Languages : English
- Source: Cryobiology - vol. 21 - n. 5
View record
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THE USE OF LOW MOLECULAR WEIGHT NON-PENETRATING...
- Author(s) : BREARLEY C. A., HODGES N. A., OLLIFF C. J.
- Date : 1990
- Languages : English
- Source: Cryo-Letters - vol. 11 - n. 4
View record
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CRYOPROTECTION OF RED BLOOD CELLS BY 1,3-BUTANE...
- Author(s) : MEHL P., BOUTRON P.
- Date : 1988
- Languages : English
- Source: Cryobiology - vol. 25 - n. 1
View record
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ERYTHROCYTES IN 1,2-BUTANEDIOL: < TOXICITY > AN...
- Author(s) : MEHL P., BOUTRON P.
- Date : 1986
- Languages : English
- Source: Cryo-Letters - vol. 7 - n. 6
View record