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16 YEAR CRYOGENIC STORAGE OF BLOOD FROZEN BY DROPLET FREEZING TECHNIQUE AND A LOW GLYCEROL-RAPID FREEZE PROCEDURE.

Author(s) : ROWE A. W.

Summary

CRYOPRESERVATION PROCEDURES FOR CELLS SUCH AS ERYTHROCYTES ARE OF LIMITED VALUE UNLESS THE TEMPERATURE OF STORAGE IS SUFFICIENTLY LOW. US REGULATIONS LIMIT STORAGE TO 3 YEARS, BUT DATA INDICATE THAT NO OUTDATING IS NECESSARY IF THE CELLS ARE STORED PROPERLY. TWO TECHNIQUES FOR RED CELL FREEZING WERE USED: (A) DROPLET FREEZING OF SMALL ALIQUOTS, PERFORMED BY SPRAYING RED CELLS SUSPENDED IN A SUGAR SOLUTION ONTO A MOVING FILM OF LIQUID NITROGEN OR (B) GLYCEROL FREEZING OF LARGE VOLUMES (500 MILLILITRES), USING THE LOW GLYCEROL-RAPID FREEZE PROCEDURE. AT 253 K (-20 DEG C), GROSS HEMOLYSIS WAS EVIDENT AT 24 HR AFTER THAWING. AT 193 K (-80 DEG C),DROPLET-FROZEN CELLS REMAINED STABLE FOR ABOUT 6 MONTHS, BUT GLYCEROL-FROZEN CELLS SHOWED EVIDENCE OF HEMOLYSIS WITHIN 1 WEEK. THESE DATA SUGGEST ICE CRYSTAL GROWTH WHICH CAUSES CELL DAMAGE AT 193 K. AT 108 AND 77 K (-165 AND -196 DEG C), DROPLET-FROZEN CELLS AND GLYCEROL-FROZEN CELLS REMAINED STABLE FOR OVER 16 YEARS.

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Details

  • Original title: 16 YEAR CRYOGENIC STORAGE OF BLOOD FROZEN BY DROPLET FREEZING TECHNIQUE AND A LOW GLYCEROL-RAPID FREEZE PROCEDURE.
  • Record ID : 1988-1681
  • Languages: English
  • Source: Development in refrigeration, refrigeration for development. Proceedings of the XVIIth international Congress of Refrigeration.
  • Publication date: 1987/08/24
  • Document available for consultation in the library of the IIR headquarters only.

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