Share

IIR document

Polydimethylsiloxane as perspective cold and heat transfer fluid with cryoprotective capabilities for the use in biobanking.

Number: 0005

Author(s) : LAUK-DUBITSKY S. E., SHAKUROV A. V., PUSHKAREV A. V., et al.

Summary

The aim of this paper is to demonstrate the new method of cryopreservation by immersion in polydimethylsiloxane (PDMS) with possible repeated freezing. More than 60 cadaveric femoral arteries (4 cm long) and 7 aortic valves (5 cm long) with preliminary mathematical modeling were cryopreserved (up to three times) in PDMS on sterile hollow plastic tubes with stirring for 2 minutes, storing at minus 80 °C and thawing in the same PDMS. The quality of cryopreservation was assessed by histological and immunohistochemical methods, scanning electron microscopy and biomechanical tests. According to morphology examinations, there ware no significant and critical differences between the segments of experimental and control groups. Using of polydimethylsiloxane is feasible for an instant, cost-effective and one-stage cryopreservation of blood vessels for their biobanking.

Available documents

Format PDF

Available

  • Public price

    20 €

  • Member price*

    15 €

* Best rate depending on membership category (see the detailed benefits of individual and corporate memberships).

Details

  • Original title: Polydimethylsiloxane as perspective cold and heat transfer fluid with cryoprotective capabilities for the use in biobanking.
  • Record ID : 30026875
  • Languages: English
  • Source: 3rd IIR Conference on cold applications in life sciences - cryotherapy and cryopreservation.
  • Publication date: 2018/09/12
  • DOI: http://dx.doi.org/10.18462/iir.CALS.2018.0005

Links


See other articles from the proceedings (1)
See the conference proceedings

Indexing