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EFFECT OF DMSO AND PROTEIN CONCENTRATION ON THE VIABILITY OF TWO-CELL MOUSE EMBRYOS FROZEN WITH A RAPID FREEZING TECHNIQUE.

Author(s) : SHAW J. M., TROUNSON A. O.

Type of article: Article

Summary

TWO-CELL MOUSE EMBRYOS WERE FROZEN BY DIRECT PLUNGING INTO LIQUID NITROGENAFTER A 3-MINUTE EXPOSURE TO SOLUTIONS CONTAINING 0.25 M SUCROSE WITH 1.5, 3, OR 4.5 M DMSO, AND 0, 4, 8, 16, OR 32 MG/ML BOVINE SERUM ALBUMIN (BSA). THE SURVIVAL RATE WAS VERY HIGH WITH 3 AND 4.5 M OF DMSO, PROVIDED BSA WAS PRESENT, THEAMOUNT OF WHICH MADE NO SIGNIFICANT DIFFERENCE.

Details

  • Original title: EFFECT OF DMSO AND PROTEIN CONCENTRATION ON THE VIABILITY OF TWO-CELL MOUSE EMBRYOS FROZEN WITH A RAPID FREEZING TECHNIQUE.
  • Record ID : 1990-1708
  • Languages: English
  • Source: Cryobiology - vol. 26 - n. 5
  • Publication date: 1989

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