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LOW TEMPERATURE PRESERVATION OF 2-CELL MOUSE EMBRYOS IN WHITTEN'S MEDIUM CONTAINING HEPES BUFFER.

[In Russian. / En russe.]

Author(s) : MEZEVIKINA L. M., BEREZOVSKAJA O. P., VEPRINCEV B. N.

Type of article: Article

Summary

THE CULTURE WHITTEN'S MEDIUM WITH HEPES BUFFER HAS BEEN USED AT SUCH STAGES OF CRYOPRESERVATION AS ISOLATION, FREEZING, CULTIVATION, AND TRANSPLANTATION. AFTER THAWING FROM 77 K (-196 DEG C), 80% OF 2-CELL EMBRYOS OF MICE F1 HAD NORMAL MORPHOLOGY, 60% OF NORMAL EMBRYOS DEVELOPED IN VITRO FROM 2-CELL STAGE TO BLASTOCYSTS, AND 36% OF EMBRYOS DEVELOPED INTO NORMAL MICE IF TRANSPLANTED INTO PSEUDOPREGNANT FOSTER MOTHERS. EMBRYO DAMAGES CAUSED BY FREEZING-THAWING AND DMSO EFFECTS WERE PRONOUNCED IN THE PREIMPLANTATION PERIODS OF DEVELOPMENT.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1989-1197
  • Languages: Russian
  • Source: Kriobiologija - n. 3
  • Publication date: 1988
  • Document available for consultation in the library of the IIR headquarters only.

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