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EFFECT OF POST-THAW TREATMENT ON THE VIABILITY OF CRYOPRESERVED LAVANDULA VERA CELLS.

Author(s) : KURIYAMA A.

Type of article: Article

Summary

CULTURED LAVANDULA VERA CELLS WERE CRYOPRESERVED IN THE PRESENCE OF DMSO AND D-GLUCOSE IN LIQUID NITROGEN. CHANGES IN THE VIABILITY OF FROZEN-THAWED CELLS WERE EXAMINED. POST-THAW WASHING WITH LIQUID CULTURE MEDIUM DECREASED THE VIABILITY OF FROZEN-THAWED CELLS MARKEDLY ALTHOUGH IT WAS BENEFICIAL TO UNFROZEN-CRYOPROTECTED CELLS. ADDITION OF ACTIVATED CHARCOAL TO THE POST-THAW CULTURE MEDIUM REDUCED POST-THAW VIABILITY LOSS AND INCREASED THE FREQUENCY OF COLONY FORMATION OF THE CELLS AFTER FURTHER CULTURE.

Details

  • Original title: EFFECT OF POST-THAW TREATMENT ON THE VIABILITY OF CRYOPRESERVED LAVANDULA VERA CELLS.
  • Record ID : 1991-0873
  • Languages: English
  • Source: Cryo-Letters - vol. 11 - n. 3
  • Publication date: 1990/05
  • Document available for consultation in the library of the IIR headquarters only.

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