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SEPARATION OF COD (GADUS MORHUA) FILLET PROTEINS BY ELECTROPHORESIS AND HPLC AFTER VARIOUS FROZEN STORAGE TREATMENTS.

Author(s) : LEBLANC E. L., LEBLANC R. J.

Type of article: Article

Summary

MYOFIBRILLAR, SARCOPLASMIC (SAR) AND TOTAL EXTRACTABLE PROTEINS (TEP) WERE ISOLATED FROM THE SAME LOT OF FROZEN COD (GADUS MORHUA) FILLETS STORED AT 243, 251, 258 AND 261 K (-30, -22, -15 AND -12 DEG C) AND A SIMULATED INDUSTRIAL FLUCTUATING TEMPERATURE (SIFT) PROGRAM WAS DEVELOPED. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) AND SODIUM DODECYL SULFATE POLYACRYLAMIDE GEL ELECTROPHORESIS SDS-PAGE SHOWED THAT MYOFIBRILLAR AND SAR OF THE FILLETS STORED AT 261 AND 258 K EXHIBITED LOW AND HIGH MOLECULAR WEIGHT CHANGES SIMILAR TO THE SIFT. A NEW INDICATOR OF MYOFIBRILLAR FROZENSTORAGE QUALITY WAS DEVELOPED USING SDS-PAGE. TEP PROFILES BY HPLC WERE TIME AND TEMPERATURE DEPENDENT.

Details

  • Original title: SEPARATION OF COD (GADUS MORHUA) FILLET PROTEINS BY ELECTROPHORESIS AND HPLC AFTER VARIOUS FROZEN STORAGE TREATMENTS.
  • Record ID : 1990-1120
  • Languages: English
  • Source: Ital. J. Food Sci. - vol. 54 - n. 4
  • Publication date: 1989
  • Document available for consultation in the library of the IIR headquarters only.

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