ShareFREEZING OF SHEEP EMBRYOS IN 3.0 M METHANOL.
Author(s) : CZLONKOWSKA M.
Type of article: Article
Summary
THE SURVIVAL OF 35.7% EMBRYOS WAS OBSERVED AFTER EQUILIBRATION IN MEDIUM PB1 CONTAINING 3.0 M METHANOL, SLOW COOLED (0.5 K/MIN) TO 238 K (-35 DEG C) AND NEXT RAPIDLY WARMED (ABOUT 800 K/MIN). AFTER USING THIS SAME COOLING PROCEDURE AND DEEP FREEZING TO 77 K (-196 DEG C), THE SURVIVAL WAS ONLY SLIGHTLY LOWER (30.4%). THE RESULTS INDICATE THAT IT IS POSSIBLE TO FREEZE SHEEP EMBRYOS IN THE PRESENCE OF 3.0 M METHANOL.
Details
- Original title: FREEZING OF SHEEP EMBRYOS IN 3.0 M METHANOL.
- Record ID : 1992-0417
- Languages: English
- Source: Cryo-Letters - vol. 12 - n. 1
- Publication date: 1991
- Document available for consultation in the library of the IIR headquarters only.
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Ewe; Cryoprotectant; Methanol; Survival; Embryo; Cryopreservation
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