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Sucrose preculture to simplify cryopreservation of banana meristem cultures.

Author(s) : PANIS B., STROSSE H., HENDE S. van den, et al.

Type of article: Article

Summary

The authors describe a simple cryopreservation method for proliferating meristem cultures of banana (Musa spp.). It relies on a 2-week preculture on media containing 0.4 M sucrose followed by rapid cooling in liquid nitrogen. Different preculture media were screened for efficient protection of banana meristems during cryopreservation. Sucrose can be replaced by both fructose and glucose without significantly affecting post-thaw survival. A high BA concentration in the preculture medium results in less material available for cryopreservation, but does not affect cryoprotection. Culture in liquid media significantly improved post-thaw regeneration. It was shown that post-thaw regeneration frequencies (ranging between 0 and 66%) are highly dependent on the genomic constitution of the banana cultivar.

Details

  • Original title: Sucrose preculture to simplify cryopreservation of banana meristem cultures.
  • Record ID : 2003-3032
  • Languages: English
  • Source: CryoLetters - vol. 23 - n. 6
  • Publication date: 2002/11
  • Document available for consultation in the library of the IIR headquarters only.

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