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System of cross-contamination prevention during storage of haematopoietic progenitor cells.

Author(s) : MERICKA P., BLÁHA M., VÁVRA L., et al.

Summary

The possibility of cross-contamination among damaged plastic bags stored in the liquid phase of nitrogen and containing cells for clinical application represents an urgent issue after publication of Tedder's paper describing transmission of hepatitis B via this mechanism. The preventive measure applied by us include: 1) meticulous testing of all patients and donors; 2) bacteriological testing of collected concentrates; 3) decision on safe method of storage (liquid or vapour phase of nitrogen or mechanical freezer) on the basis of the result of the serological tests; 4) use of high-quality plastic disposable bags and external metal cover preventing mechanical damage during storage. The study performed in 93 patients and donors showed that the potential source of cross contamination is a relatively high active virus infection, herpes viruses being the most common finding. Bacterial contamination occurred only in 0.42% of 479 bags tested for sterility and represents a negligible risk in the centre.

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Details

  • Original title: System of cross-contamination prevention during storage of haematopoietic progenitor cells.
  • Record ID : 2004-3036
  • Languages: English
  • Source: 21st IIR International Congress of Refrigeration: Serving the Needs of Mankind.
  • Publication date: 2003/08/17

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