Share

The current status of tissue cryopreservation.

Author(s) : PEGG D. E.

Type of article: Article

Summary

For certain tissues, living and functioning cells are needed and preservation methods need perfecting. The basic requirements for cell recovery can usually be defined if a few basic biophysical properties of the cell are known and some standard measurements of the effect of cryobiological variables are carried out. Problems in tissue cryopreservation are not usually due to difficulties in preserving the living cells, but arise from the properties of the integrated cell/matrix systems upon which tissue function usually depends. The formation of ice is probably fundamental to these difficulties, and this is why vitrification seems the most likely way forward. Two major problems to be overcome are cryoprotectant toxicity and recrystallization during rewarming. Chilling injury (damage caused by reduction in temperature per se) may be of fundamental importance.

Details

  • Original title: The current status of tissue cryopreservation.
  • Record ID : 2002-0520
  • Languages: English
  • Source: CryoLetters - vol. 22 - n. 2
  • Publication date: 2001/03
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (2)
See the source

Indexing