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THE EFFECT OF INITIAL TONICITY ON FREEZE/THAW INJURY TO HUMAN RED CELLS SUSPENDED IN SOLUTIONS OF SODIUM CHLORIDE.

Author(s) : PEGG D. E., DIAPER M. P.

Type of article: Article

Summary

HUMAN RED BLOOD CELLS, SUSPENDED IN SOLUTIONS OF SODIUM CHLORIDE, WERE FROZEN TO TEMPERATURES BETWEEN 271 AND 259 K (-2 AND -14 DEG C) AND THAWED, AND THE EXTENT OF HEMOLYSIS WAS MEASURED. IN PARALLEL EXPERIMENTS, RED CELLS WERE EXPOSED TO SIMILAR CYCLES OF CHANGE IN THE COMPOSITION OF THE SUSPENDING SOLUTION, BUT BY DIALYSIS AT 294 K (21 DEG C). IT WAS FOUND THAT THE TONICITY OF THE SALINE USED TO SUSPEND THE CELLS FOR THE MAIN BODY OF THE EXPERIMENT AFFECTED THE AMOUNTOF HEMOLYSIS MEASURED.

Details

  • Original title: THE EFFECT OF INITIAL TONICITY ON FREEZE/THAW INJURY TO HUMAN RED CELLS SUSPENDED IN SOLUTIONS OF SODIUM CHLORIDE.
  • Record ID : 1991-2783
  • Languages: English
  • Source: Cryobiology - vol. 28 - n. 1
  • Publication date: 1991/02

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