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THE PRESERVATION OF CONTRACTILE PROTEINS DURING STORAGE OF HUMAN PLATELETS.

Author(s) : LUCAS R. C., LAWRENCE J., STRACHER A.

Type of article: Article

Summary

PLATELET CONCENTRATES WERE STORED AT 277 AND 298K (4 AND 25 DEGC) FOR UP TO 14 DAYS, AND THEIR ABILITY TO SECRETE AND AGGREGATE IN RESPONSE TO APPROPRIATE STIMULI WAS DETERMINED AT 6, 96, AND 192 HR AFTER VENIPUNCTURE. AT EACH TIME POINT THE PROTEIN COMPLEMENT OF THE PLATELETS WAS ALSO MONITORED TO ASSESS THE EXTENT OF INTRACELLULAR PROTEIN DEGRADATION. PLATELETS FROM CONCENTRATES STORED AT EITHER TEMPERATURE EXHIBITED A DECREASED ABILITY TO RESPOND TO STIMULI AS STORAGE TIME INCREASED. AFTER 8 DAYS OF STORAGE AT 277 K AND UP TO 9 DAYS AT 298K, NO PROTEOLYSIS OF MAJOR PLATELET PROTEINS WAS OBSERVED; HOWEVER, COMPLETE LOSS OF PLATELET FUNCTION WAS OBSERVED. THIS STRONGLY INDICATES THAT A DECREASE IN PLATELET FUNCTION SHOULD NOT BE CAUSALLY LINKED TO DEGRADED CONTRACTILE-STRUCTURAL PROTEINS AND THAT EXTENDING THE FUNCTIONAL LIFE OF PLATELETS DURING STORAGE IS STILL AN ATTAINABLE GOAL SINCE PROTEOLYSIS IS NOT THE INEVITABLE RESULT OF SHORT-TERM STORAGE.

Details

  • Original title: THE PRESERVATION OF CONTRACTILE PROTEINS DURING STORAGE OF HUMAN PLATELETS.
  • Record ID : 1982-1749
  • Languages: English
  • Source: Blood - vol. 57 - n. 6
  • Publication date: 1981
  • Document available for consultation in the library of the IIR headquarters only.

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