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Thermal analysis of lyoprotectants for biomaterials.

Author(s) : ZHOU X. L., TAO H., ZHANG S. Z., et al.

Summary

Glass transition temperatures of lyoprotectants for biomaterials are important for choosing lyoprotectants, designing freeze-drying processes and choosing storage conditions. Trehalose, lactose, maltose, sucrose, glucose and bovine serum albumin are chosen as lyoprotectants for biomaterials in this study. Differential scanning calorimetry was employed to measure the glass transition temperatures of suspension and freeze-dried sample. The factors that influenced the glass transition temperatures of suspension and freeze-dried sample were investigated. The addition of bovine serum albumin increased the glass transition temperatures of suspension and freeze-dried sample. The glass transition temperature of suspension increased with trehalose concentration, while the glass transition temperature of freeze-dried sample was almost not affected by solution concentration. The glass transition temperatures of suspension and freeze-dried sample of trehalose, lactose and maltose are high and close to each other, then the glass transition temperature of sucrose is a little lower, and that of glucose is far lower than those of other sugars. Trehalose, lactose and maltose are more suitable to act as lyoprotectants than sucrose and glucose. Except for the formulation of 20% glucose, the lowest glass transition temperature of suspension is -36.2°C, the lowest glass transition temperature of freeze-dried sample is 35.3°C. According to those results, the freezing temperature is determined at -40°C, the drying temperature is determined at -38°C and the storage temperature is determined at room temperature below 30°C during freeze-drying of biomaterials.

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Pages: ICR07-C1-945

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Details

  • Original title: Thermal analysis of lyoprotectants for biomaterials.
  • Record ID : 2008-0954
  • Languages: English
  • Source: ICR 2007. Refrigeration Creates the Future. Proceedings of the 22nd IIR International Congress of Refrigeration.
  • Publication date: 2007/08/21

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