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TOLERANCE OF HUMAN CORNEAL ENDOTHELIUM TO GLYCEROL.

Author(s) : BRUNETTE I., NELSON L. R., BOURNE W. M.

Type of article: Article

Summary

AS AN INITIAL STEP IN THE DEVELOPMENT OF A METHOD FOR CORNEAL CRYOPRESERVATION BY VITRIFICATION, THE AUTHORS ATTEMPTED TO ESTABLISH THE MAXIMUM CONCENTRATION OF GLYCEROL TO WHICH HUMAN CORNEAL ENDOTHELIUM COULD BE EXPOSED AT 277 K (4 DEG C) FOR 15 MIN WITHOUT DAMAGE. DAMAGE WAS DEFINED AS AN INCREASE IN MEAN ENDOTHELIAL CELL SIZE. THE MEAN ENDOTHELIAL CELL SIZE INCREASED IN CORNEAS EXPOSED TO GLYCEROL CONCENTRATIONS OF 40, 50, AND 60% (W/V), BUT DID NOT DIFFER SIGNIFICANT-LY FROM BASELINE MEASUREMENTS IN THE CORNEAS EXPOSED TO 30% GLYCEROL OR LESS. TOLERANCE OF HIGHER CONCENTRATIONS MAY BE ACHIEVED BY CHANGES IN THE RATES OF ADDITION AND REMOVAL OF GLYCEROL OR IN THE COMPOSITION OF THE PERFUSATE.

Details

  • Original title: TOLERANCE OF HUMAN CORNEAL ENDOTHELIUM TO GLYCEROL.
  • Record ID : 1990-1706
  • Languages: English
  • Source: Cryobiology - vol. 26 - n. 6
  • Publication date: 1989

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