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Viability analysis of cryopreserved rat pancreatic islets using laser scanning confocal microscopy.

Author(s) : MERCHANT F. A., DILLER K. R., AGGARWAL S. J., BOVIK A. C.

Type of article: Article

Summary

The studies indicate that cryopreserved islets exhibit shape distortion and a decrease in the numerical density of cells in comparison to unfrozen controls. Maximal survival was observed at the slower cooling rates. Accordingly, damage was found to occur throughout the 3-D islet volume in distinct spatial distribution for islets frozen at the slower and the faster cooling rates. Further, it was found that the volume of the majority of damaged cells identified was consistent with that of cells ranging in diameter from 5 to 9 micrometers.

Details

  • Original title: Viability analysis of cryopreserved rat pancreatic islets using laser scanning confocal microscopy.
  • Record ID : 1997-0552
  • Languages: English
  • Source: Cryobiology - vol. 33 - n. 2
  • Publication date: 1996/04

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