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VITRIFICATION OF HUMAN ISLETS OF LANGERHANS.

Author(s) : JUTTE N. H. P. M.

Type of article: Article

Summary

ISOLATED ISLETS WERE DIVIDED INTO 4 GROUPS: CONTROL ISLETS WHICH WERE CULTURED EITHER FOR 6, OR FOR 10 TO 13 DAYS, ISLETS VITRIFIED AFTER 2 DAYS OF CULTURE AND ISLETS VITRIFIED AFTER 6-9 DAYS OF CULTURE. THE THUS TREATED ISLETS WERE INVESTIGATED WITH RESPECTTO INSULIN SECRETION IN THE PRESENCE OF 2.5 OR 25 MILLIMOLES GLUCOSE, CAPACITY TO SURVIVE DURING POSTWARMING CULTURE, AND MORPHOLOGY. THE INSULIN SECRETION IN ISLETS FROM ALL GROUPS COULD BE STIMULATED BY AN INCREASE OF THE CONCENTRATIONOF GLUCOSE FROM 2.5 TO 25 MILLIMOLES. NO SIGNIFICANT DIFFERENCES WERE OBSERVED BETWEEN THE INSULIN SECRETIONS OF THE VITRIFIED AND CONTROL ISLETS.

Details

  • Original title: VITRIFICATION OF HUMAN ISLETS OF LANGERHANS.
  • Record ID : 1988-0829
  • Languages: English
  • Source: Cryobiology - vol. 24 - n. 5
  • Publication date: 1987

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