Share

CRYOPRESERVATION OF MOUSE PANCREATIC ISLETS: EFFECTS OF DIFFERENT GLUCOSE CONCENTRATIONS IN THE POST-THAW CULTURE MEDIUM ON ISLET RECOVERY.

Author(s) : SANDLER S., ANDERSSON A.

Type of article: Article

Summary

STUDY OF THE INFLUENCE OF THE GLUCOSE CONCENTRATION OF THE POST-THAW CULTURE MEDIUM ON ISLET B-CELL SURVIVAL AFTER CRYOPRESERVATION BY THECOMBINED ASSESSMENTS OF ISLET RECOVERY, ISLET DNA AND INSULIN CONTENTS, AND INSULIN RELEASE. COLLAGENASE ISOLATED MOUSE ISLETS WERE KEPT IN CULTURE FOR 3 DAYS IN THE PRESENCE OF 11.1 MILLIMOLES GLUCOSE AND THEN TRANSFERRED TO FREEZING AMPOULES CONTAINING HANK'S SOLUTION SUPPLEMENTED WITH 10% CALF SERUM AND 2 M DMSO. THE PERCENTAGE RECOVERY OF CRYOPRESERVED ISLETS WAS DECREASED COMPARED TO THAT OF NONFROZEN ISLETS, BUT WAS INCREASED WHEN HIGHER GLUCOSE CONCENTRATIONS WERE USED IN THE POST-THAW CULTURE MEDIUM. THE PRESENT FINDINGS ALSO SUGGEST THAT A HIGH GLUCOSE CONCENTRATION IS NOT DELETERIOUS TO CRYOPRESERVED ISLETS. RATHER THE ISLET RECOVERY WAS ENHANCED BY GLUCOSE, PRESUMABLY BECAUSE OF ITS ENERGY GENERATION IN THE B CELLS, AND THIS MAY ALLOW REPAIR OF SMALL INJURIES INDUCED BY THE FREEZE-THAWING.

Details

  • Original title: CRYOPRESERVATION OF MOUSE PANCREATIC ISLETS: EFFECTS OF DIFFERENT GLUCOSE CONCENTRATIONS IN THE POST-THAW CULTURE MEDIUM ON ISLET RECOVERY.
  • Record ID : 1989-0358
  • Languages: English
  • Source: Cryobiology - vol. 24 - n. 4
  • Publication date: 1987

Links


See other articles in this issue (6)
See the source

Indexing