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VITRIFICATION OF MOUSE ISLETS OF LANGERHANS: COMPARISON WITH A MORE CONVENTIONAL FREEZING METHOD.

Author(s) : JUTTE N. H. P. M.

Type of article: Article

Summary

THE POSSIBILITY OF CRYOPRESERVATION OF ISLETS OF LANGERHANS BY VITRIFICATION USING A MIXTURE OF CRYOPROTECTANTS WAS INVESTIGATED AND THE RESULTS WERE COMPARED WITH A MORE CONVENTIONAL FREEZING METHOD USING DMSO AS CRYOPROTECTANT. THE SURVIVAL OF ISLETS DURING POSTWARMING CULTURE WAS COMPARABLE AFTER CRYOPRESERVATION WITH BOTH METHODS, AND ISLETS FROM BOTH GROUPS COULD LOWER SERUM GLUCOSE STREPTOZOTOCIN DIABTCC. THE AUTHORS CONCLUDE THAT ISLETS CRYOPRESERVED BY THE VITRIFICATION METHOD ARE FUNCTIONALIN VITRO AND IN VIVO. THIS METHOD IS QUICK, SIMPLE, AND CHEAP BECAUSE THE USE OF COMPLICATED FREEZING EQUIPMENT IS AVOIDED.

Details

  • Original title: VITRIFICATION OF MOUSE ISLETS OF LANGERHANS: COMPARISON WITH A MORE CONVENTIONAL FREEZING METHOD.
  • Record ID : 1989-0359
  • Languages: English
  • Source: Cryobiology - vol. 24 - n. 4
  • Publication date: 1987

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