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Water in animal cells and cryopreservation: development to medical field.

[In Japanese. / En japonais.]

Author(s) : TAKADA K.

Type of article: Article

Summary

Animal cells are 90% composed of water, and the regulation of water during freezing is critical for their successful cryopreservation. Contrary to frozen foods, cells should retain their functional and developmental capacity after a period of storage. Two kinds of cryoprotectants, membrane permeable and non-permeable cryoprotectants were found in 1950s, and their mechanisms of cryoprotection have been extensively studied. A vitrification method was developed to cryopreserve human organs, and the method has been applied to the storage of animal embryos. In the medical field, cord blood banking is a typical example that requires cryopreservation. The medium to cryopreserve cord blood was developed using a combination of membrane permeable and non-permeable cryoprotectants, and the freezing procedure is fully automated through LN2 storage. Successful cryopreservation of these cells guarantees the lives of transplanted patients.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 2004-1974
  • Languages: Japanese
  • Source: Refrigeration - vol. 79 - n. 915
  • Publication date: 2004
  • Document available for consultation in the library of the IIR headquarters only.

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