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A dialysis-washing system for removal of cryoprotective agent from cryopreserved human red blood cells.

Author(s) : ZHANG X. G., ZHAO G., DING W. P., et al.

Summary

In cryopreservation of human red blood cells (RBCs), a cryoprotective agent glycerol (40% w/v) must be used to prevent cells from cryoinjury. However, the cryoprotectant needs to be removed from the cells after thawing because of the toxicity of glycerol to the cells at room or body temperatures. Furthermore, when the cells are transfused into a patient's body if glycerol is not removed, the high osmolality will cause great osmotic shock to the cells in the body. Currently, glycerol is removed using a procedure involving centrifugation, which introduces mechanical forces and osmotic stress causing cell packing/clumping and significant cell loss. Besides, the currently used method is an open system, which may cause bacterial contamination. To prevent cell clumping, cell loss, and osmotic injury, a novel diffusion-washing device (without centrifugation) is developed to remove glycerol. Similar to hemodialysis process, the perfusion-washing system is composed of a mini-module dialyzer, blood side tubings and dialysate side tubings. Results show that the dialysis-washing method can remove the cryoprotective agent in about 30 minutes. The count recovery and haemoglobin recovery of the RBCs is about 90 and 85%.

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Pages: ICR07-C1-1508

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Details

  • Original title: A dialysis-washing system for removal of cryoprotective agent from cryopreserved human red blood cells.
  • Record ID : 2008-0930
  • Languages: English
  • Source: ICR 2007. Refrigeration Creates the Future. Proceedings of the 22nd IIR International Congress of Refrigeration.
  • Publication date: 2007/08/21

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