Summary
The effect of rapid freeze-thawing on the structural rearrangement of integral and peripheral membrane proteins was studied using fluorescent spectroscopy. The data are interpreted in terms of physical and chemical factors in the medium affecting the spectral properties of protein chromophores, and suggest that an increase occurs in the polarity of the microenvironment of the protein molecules, resulting in conformational modification.
Details
- Original title: A comparative study of the effect of freeze-thawing on peripheral and integral membrane proteins.
- Record ID : 1998-1288
- Languages: English
- Source: Cryobiology - vol. 34 - n. 2
- Publication date: 1997/03
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cell; Protein; Membrane; Freezing-thawing
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ISOLATION AND PARTIAL PURIFICATION OF CRYOPROTE...
- Author(s) : HINCHA D. K., HEBER U., SCHMITT J. M.
- Date : 1989/07/06
- Languages : English
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EXAMINATION OF STRUCTURAL CHANGES IN ERYTHROCYT...
- Author(s) : LOYEVSKY M. M., GUSEVA N. R., BELOUS A. M.
- Date : 1987
- Languages : English
- Source: Cryo-Letters - vol. 8 - n. 3
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INVESTIGATION OF THE MEMBRANE INTEGRITY OF HUMA...
- Author(s) : GAVRILOVA I. I., IVANOV L. V., MOISEYEV V. A.
- Date : 1981
- Languages : English
- Source: Cryo-Letters - vol. 2 - n. 7
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EFFECT OF FREEZING-THAWING ON THE HUMAN PLATELE...
- Author(s) : KRALISZ U., SUREWICZ W. K., KOTELBA-WITKOWSKA B.
- Date : 1985
- Languages : English
- Source: Cryo-Letters - vol. 6 - n. 1
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INVESTIGATION OF REPARATION OF MITOCHONDRIAL ME...
- Author(s) : PETRENKO A. Ju.
- Date : 1987
- Languages : Russian
- Source: Kriobiologija - n. 2
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