ShareSummary
The effect of rapid freeze-thawing on the structural rearrangement of integral and peripheral membrane proteins was studied using fluorescent spectroscopy. The data are interpreted in terms of physical and chemical factors in the medium affecting the spectral properties of protein chromophores, and suggest that an increase occurs in the polarity of the microenvironment of the protein molecules, resulting in conformational modification.
Details
- Original title: A comparative study of the effect of freeze-thawing on peripheral and integral membrane proteins.
- Record ID : 1998-1288
- Languages: English
- Source: Cryobiology - vol. 34 - n. 2
- Publication date: 1997/03
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cell; Protein; Membrane; Freezing-thawing
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INVESTIGATION OF REPARATION OF MITOCHONDRIAL ME...
- Author(s) : PETRENKO A. Ju.
- Date : 1987
- Languages : Russian
- Source: Kriobiologija - n. 2
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YEAST FREEZE-THAW SURVIVAL RATES AS A FUNCTION ...
- Author(s) : COTTRELL S. F.
- Date : 1981
- Languages : English
- Source: Cryobiology - vol. 18 - n. 5
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CHANGES IN THE PERMEABILITY CHARACTERISTICS OF ...
- Author(s) : FULLER B. J., LOECKER W. de
- Date : 1985
- Languages : English
- Source: Cryo-Letters - vol. 6 - n. 6
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EXAMINATION BY FLUORESCENCE AND EPR SPECTROSCOP...
- Author(s) : BELOUS A. M.
- Date : 1990
- Languages : English
- Source: Cryobiology - vol. 27 - n. 2
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MANIFESTATIONS OF CELL DAMAGE AFTER FREEZING AN...
- Author(s) : MCGANN L. E., YANG H., WALTERSON M.
- Date : 1988
- Languages : English
- Source: Cryobiology - vol. 25 - n. 3
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