A comparative study of the effect of freeze-thawing on peripheral and integral membrane proteins.

Author(s) : NARDID O., DYUBKO T., REPINA S.

Type of article: Article

Summary

The effect of rapid freeze-thawing on the structural rearrangement of integral and peripheral membrane proteins was studied using fluorescent spectroscopy. The data are interpreted in terms of physical and chemical factors in the medium affecting the spectral properties of protein chromophores, and suggest that an increase occurs in the polarity of the microenvironment of the protein molecules, resulting in conformational modification.

Details

  • Original title: A comparative study of the effect of freeze-thawing on peripheral and integral membrane proteins.
  • Record ID : 1998-1288
  • Languages: English
  • Source: Cryobiology - vol. 34 - n. 2
  • Publication date: 1997/03

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