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YEAST FREEZE-THAW SURVIVAL RATES AS A FUNCTION OF DIFFERENT STAGES IN THE CELL CYCLE.

Author(s) : COTTRELL S. F.

Type of article: Article

Summary

THE STUDY DEMONSTRATES THAT THE 193K (-80 DEGC) FREEZE-THAW SURVIVAL RATE IN THE YEAST, SACCHAROMYCES CEREVISIAE, IS DEPENDENT UPON SPECIFIC STAGES IN THE CELL CYCLE. SAMPLES FROM CELL CYCLES WERE SUBJECTED TO A 193K FREEZE-THAW PROTOCOL EMPLOYING 10% GLYCEROL AS A CRYOPROTECTANT. MAXIMUM RATES OF SURVIVAL OCCURRED AT THE INITIATION OF EACH NEW CELL CYCLE AND MINIMUM RATES OF SURVIVAL OCCURRED APPROXIMATELY 30 MIN PRIOR TO EACH NEW CELL CYCLE. THESE RATES WERE SHOWN TO BE CORRELATED IN TIME WITH MAXIMUM AND MINIMUM RATIOS OF CELLULAR PHOSPHOLIPID TO MEMBRANE DURING EACH INDIVIDUAL CELL CYCLE.

Details

  • Original title: YEAST FREEZE-THAW SURVIVAL RATES AS A FUNCTION OF DIFFERENT STAGES IN THE CELL CYCLE.
  • Record ID : 1982-1744
  • Languages: English
  • Source: Cryobiology - vol. 18 - n. 5
  • Publication date: 1981

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