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A COMPARATIVE STUDY OF THE EFFECTS OF FREEZING AND FROZEN STORAGE ON INTACT AND DEMEMBRANATED BULL SPERMATOZOA.

Author(s) : LINDEMANN C. B., FISHER M., LIPTON M.

Type of article: Article

Summary

THE DAMAGE CAUSED TO BULL SPERM BY FREEZING AND THAWING THEM WITHOUT CRYOPROTECTANTS WAS ASSESSED IN BOTH INTACT AND MEMBRANE-EXTRACTED CELLS. APPARENTLY FREEZING AND THAWING SPERM CELLS CAUSES DAMAGE TO THE PLASMA MEMBRANE WHICH PERMITS ATP AND THE ION MAGNESIUM TO FREELY ENTER OR LEAVE THE CELLS, BUT DOES NOT DAMAGE THE COMPONENTS OF THE SPERM CELL WHICH GENERATE MOTILITY. THE EFFECTS OF STORAGE TEMPERATURE ON FROZEN DEMEMBRANATED SPERM WERE ALSO EXPLORED. SPERM HELD AT 253 K (-20 DEG C) SHOWED MARKED STRUCTURAL CHANGES AND PROGRESSIVELY DECREASED MOTILITY AFTER PROLONGED STORAGE.

Details

  • Original title: A COMPARATIVE STUDY OF THE EFFECTS OF FREEZING AND FROZEN STORAGE ON INTACT AND DEMEMBRANATED BULL SPERMATOZOA.
  • Record ID : 1982-2113
  • Languages: English
  • Source: Cryobiology - vol. 19 - n. 1
  • Publication date: 1982

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