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A custom-built controlled-rate freezer for small sample cryopreservation studies.

Author(s) : MEDRANO A., ANDERSON W. J., MILLAR J. D., et al.

Type of article: Article

Summary

A custom-built controlled-rate freezing machine is described. It operates by automatically raising and lowering the sample carrier in a static column of liquid nitrogen vapour, and is controlled by a computer-assisted thermocouple feedback system that operates a stepper motor driving the sample carrier. The cooling protocol is divided into three phases: cooling from 5 to -5 °C, initiation of ice nucleation, and cooling from -5 to -80 °C. Experimental validation of the device over a range of different cooling rates is described. A freezing protocol is established to cool samples in plastic straws over a range of rates up to 80 °C/min, with rapid and consistent absorption of the latent heat.

Details

  • Original title: A custom-built controlled-rate freezer for small sample cryopreservation studies.
  • Record ID : 2003-3016
  • Languages: English
  • Source: CryoLetters - vol. 23 - n. 6
  • Publication date: 2002/11
  • Document available for consultation in the library of the IIR headquarters only.

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