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A NOTE ON THE MULTIPLE ROLE OF ARTIFICIAL NUCLEATION OF THE SUSPENDING MEDIUM, DURING TWO-STEP CRYOPRESERVATION PROCEDURES.

Author(s) : KEEFE P. D., HENSHAW G. G.

Type of article: Article

Summary

IT WAS OBSERVED THAT FAILURE TO NUCLEATE THE CRYOPROTECTANT MEDIUM SURROUNDING AXILLARY SHOOT TIP EXPLANTS OF SOLANUM TUBEROSUM AT 268 K (-5 DEG C), RESULTED IN TOTAL SAMPLE MORTALITY AT 253 TO 248 K (-20 TO -25 DEG C). AT 253 K SAMPLES REMAINED UNHARMED IF THE SUSPENDING MEDIUM REMAINED SUPERCOOLED, SUSTAINED ONLY SLIGHT INJURY IF FREEZING WAS INDUCED AT 268 K PRIOR TO COOLING. THIS RESULT DEMONSTRATES THE BENEFICIAL EFFECT OF ARTIFICIAL NUCLEATION OF THE SUSPENDING MEDIUM AT A TEMPERATURE CLOSE TO ITS FREEZING POINT, AND SUGGESTS THE OPERATION OF A MECHANISM OF FREEZING INJURY -INOCULATIVE INTRACELLULAR FREEZING -NOT GENERALLY ACKNOWLEDGED IN PLANT CRYOPRESERVATION STUDIES.

Details

  • Original title: A NOTE ON THE MULTIPLE ROLE OF ARTIFICIAL NUCLEATION OF THE SUSPENDING MEDIUM, DURING TWO-STEP CRYOPRESERVATION PROCEDURES.
  • Record ID : 1984-2291
  • Languages: English
  • Source: Cryo-Letters - vol. 5 - n. 2
  • Publication date: 1984
  • Document available for consultation in the library of the IIR headquarters only.

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