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INFLUENCE OF DAYS OF CULTURE ON CRYOPROTECTANT-SUPPLEMENTED MEDIUM AND OF TERMINAL FREEZING TEMPERATURE ON THE SURVIVAL OF CRYOPRESERVED PEA SHOOT TIPS.

Author(s) : MCADAMS S., RATNASABAPATHI D., SMITH R. A.

Type of article: Article

Summary

PEA (PISUM SATIVUM L. CV EARLY ALASKA) SHOOT TIPS WERE CULTURED ON SOLID B5 PEA SHOOT MEDIUM CONTAINING 5 % DMSO. THE TIME THAT THE SHOOT TIPS REMAINED ON THE CRYOPROTECTANT-SUPPLEMENTED MEDIUM PRIOR TO FREEZING WAS VARIED (0 TO 4 DAYS). PERCENTAGE SURVIVAL WAS EVALUATED AT 263, 253, 243 AND 233 K (-10, -20, -30 AND -40 DEG C), WITH AND WITHOUT IMMERSION IN LIQUID NITROGEN, USING THREE-WAY FACTORIAL ANALYSES OF VARIANCE. FOR SHOOT TIPS NOT IMMERSED IN LIQUID NITROGEN, NUMBER OF DAYS OF CULTURE DID NOT INFLUENCE SURVIVAL, WHEREAS TERMINAL FREEZING TEMPERATURE DID HAVE AN EFFECT. OPTIMUM SURVIVAL WAS AT 263 AND 253 K. FOR SHOOT TIPS IMMERSED IN LIQUID NITROGEN, TERMINAL FREEZING TEMPERATURE HAD NO EFFECT, WHEREAS NUMBER OF DAYS OF CULTURE DID INFLUENCE SURVIVAL. THE LARGEST NUMBER OF SURVIVORS WAS OBTAINED AFTER 2 DAYS OF CULTURE.

Details

  • Original title: INFLUENCE OF DAYS OF CULTURE ON CRYOPROTECTANT-SUPPLEMENTED MEDIUM AND OF TERMINAL FREEZING TEMPERATURE ON THE SURVIVAL OF CRYOPRESERVED PEA SHOOT TIPS.
  • Record ID : 1992-1161
  • Languages: English
  • Source: Cryobiology - vol. 28 - n. 3
  • Publication date: 1991/06

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