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CRYOPRESERVATION OF MUSA SUSPENSION CULTURES AND SUBSEQUENT REGENERATION OF PLANTS.

Author(s) : PANIS B. J., WITHERS L. A., LANGHE E. A. L. de

Type of article: Article

Summary

THE FOLLOWING METHOD HAS BEEN USED: SLOW FREEZING AT A RATE OF 1 K/MIN TO 233 K (-40 DEG C) FOLLOWED BY PLUNGING THE CULTURES INTO LIQUID NITROGEN. RAPID THAWING TAKES PLACE IN A WATER BATH AT 313 K (40 DEG C). OF A RANGE OF CONCENTRATIONS OF DMSO AND GLYCEROL, DMSO AT 5% GAVE THE MOST EFFECTIVE CRYOPROTECTION, RESULTING IN 64% REGROWTH OF CELLS AND CELL CLUMPS. INITIATION OF ICE CRYSTALLIZATION BY SEEDING INCREASED THE REGROWTH RATE TO 92%. ONLY UNORGANISED EMBRYOGENIC AGGREGATES SURVIVE FREEZING. REGROWN SUSPENSION CULTURES ARE REGENERATED INTO NORMAL PLANTS THROUGH SOMATIC EMBRYOGENESIS.

Details

  • Original title: CRYOPRESERVATION OF MUSA SUSPENSION CULTURES AND SUBSEQUENT REGENERATION OF PLANTS.
  • Record ID : 1991-1186
  • Languages: English
  • Source: Cryo-Letters - vol. 11 - n. 5
  • Publication date: 1990
  • Document available for consultation in the library of the IIR headquarters only.

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