ShareEffects of freezing in liquid nitrogen on the properties of a soybean (Glycine max L. var. acme) callus strain used as a bioassay for cytokinin activity.
Author(s) : ENGELMANN F.
Type of article: Article
Summary
A soybean callus strain could withstand cryopreservation after a 14 to 48 hours pregrowth with 1 to 1.5 M sucrose followed by slow freezing at 0.5 deg C/min to -40 deg C before immersion in liquid nitrogen. Cryopreservation did not modify the growth rate of the strain on media containing various concentrations of kinetin and 2-isopentenyl-adenine.
Details
- Original title: Effects of freezing in liquid nitrogen on the properties of a soybean (Glycine max L. var. acme) callus strain used as a bioassay for cytokinin activity.
- Record ID : 1994-0940
- Languages: English
- Source: Cryo-Letters - vol. 13 - n. 5
- Publication date: 1992/09
- Document available for consultation in the library of the IIR headquarters only.
Links
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Indexing
- Themes: Seeds and plants
- Keywords: Plant (botany); Cryoprotectant; Liquid nitrogen; Survival; Soya; Cryopreservation
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Effects of pregrowth and freezing conditions on...
- Author(s) : BAGNIOL S., ENGELMANN F.
- Date : 1991/09
- Languages : English
- Source: Cryo-Letters - vol. 12 - n. 5
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In vitro conservation of horticultural species.
- Author(s) : ENGELMANN F.
- Date : 1991/12
- Languages : English
- Source: Acta Hortic. - n. 298
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Vitrification of Oryza sativa L. cell suspensions.
- Author(s) : WATANABE K., STEPONKUS P. L.
- Date : 1995/09
- Languages : English
- Source: Cryo-Letters - vol. 16 - n. 5
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Cryopreservation of cell suspensions of Citrus ...
- Author(s) : AGUILAR M. E., ENGELMANN F., MICHAUX-FERRIERE N.
- Date : 1993/07
- Languages : English
- Source: Cryo-Letters - vol. 14 - n. 4
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Survival strategies of plant tissues in liquid ...
- Author(s) : MATSUMOTO T.
- Date : 1996/11
- Languages : Japanese
- Source: Refrigeration - vol. 71 - n. 829
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