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A novel preculturing for enhancing the survival of in vitro-grown meristems of wasabi (Wasabia japonica) cooled to -196 deg C by vitrification.

Author(s) : MATSUMOTO T., SAKAI A., NAKO Y.

Type of article: Article

Summary

The preculture with a mixture of 0.3 mole sucrose and 0.5 mole glycerol for 16 hours at 25 deg C without subsequent cryoprotection produced a very high level of shoot formation, as high as about 85%. Thus, this preculture method significantly simplified the vitrification procedure. It was also observed that the contents of sucrose and glycerol along with proline greatly increased in the precultured meristems.

Details

  • Original title: A novel preculturing for enhancing the survival of in vitro-grown meristems of wasabi (Wasabia japonica) cooled to -196 deg C by vitrification.
  • Record ID : 1999-1240
  • Languages: English
  • Source: Cryo-Letters - vol. 19 - n. 1
  • Publication date: 1998/01
  • Document available for consultation in the library of the IIR headquarters only.

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