Share

An approach to enhance dehydration tolerance of alginate-coated dried meristems cooled to -196 deg C.

Author(s) : MATSUMOTO T., SAKAI A.

Type of article: Article

Summary

Cold-hardened, precultured meristems of lily with 0.3 M sucrose for 1 day were encapsulated in alginate gel beads including 0.3 M sucrose and then treated with a mixture of 0.8 M sucrose plus 1 M glycerol for 16 hours before dehydration. The encapsulated dried meristems cooled to -196 deg C produced a very high level (about 90%) of shoot formation. This revised procedure may expand the applicability of encapsulation-dehydration technique to less dehydration resistant materials.

Details

  • Original title: An approach to enhance dehydration tolerance of alginate-coated dried meristems cooled to -196 deg C.
  • Record ID : 1996-2273
  • Languages: English
  • Source: Cryo-Letters - vol. 16 - n. 5
  • Publication date: 1995/09
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (5)
See the source

Indexing