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Cryopreservation of in-vitro-grown apical meristems of wasabi (Wasabia japonica) by encapsulation-vitrification method.

Author(s) : MATSUMOTO T., SAKAI A., TAKAHASHI C., YAMADA K.

Type of article: Article

Summary

Alginate-coated apical meristems from in-vitro-grown wasabi were successfully cryopreserved following dehydration by vitrification solutions. Excised meristems precultured on solidified 1/2 MS medium (half-strength of ammonium nitrate and potassium nitrate) containing 0.3 M sucrose at 20 deg C for 1 day were trapped into alginate-coated beads containing a mixture of 2 M glycerol plus 0.4 M sucrose. These encapsulated meristems were dehydrated with a highly concentrated vitrification solution for about 30 min at 25 deg C or 70 to 100 min at 0 deg C prior to a plunge into liquid nitrogen. The encapsulation-vitrification method was found to be promising for cryopreserving meristems.

Details

  • Original title: Cryopreservation of in-vitro-grown apical meristems of wasabi (Wasabia japonica) by encapsulation-vitrification method.
  • Record ID : 1996-1621
  • Languages: English
  • Source: Cryo-Letters - vol. 16 - n. 4
  • Publication date: 1995/07
  • Document available for consultation in the library of the IIR headquarters only.

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