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An improved cryopreservation protocol for pineapple apices using encapsulation-vitrification.

Author(s) : GÁMEZ-PASTRANA R., MARTÍNEZ-OCAMPO Y., BERISTAIN C. I., et al.

Type of article: Article

Summary

Several modifications to the cryogenic protocols previously described for pineapple apices were performed using vitrification and encapsulation-vitrification. Pregrowth of apices in sucrose-proline before loading significantly reduced the exposure duration to PVS2 and PVS3 required for successful cryopreservation. Encapsulation and treatments with PVS3 at 0 °C gave the highest survival before and after cooling. Optimal conditions involved the encapsulation of pineapple apices in calcium alginate (3%) followed by 2 day preculture in liquid medium with 0.16 M sucrose + 0.3 M proline for 24 h and then transfer to 0.3 M sucrose + 0.3 M proline for an additional 24 h. After preculture, samples were loaded in 0.75 M sucrose + 1 M glycerol solution at room temperature (25 min.) and dehydrated with PVS3 at 0 °C for 60 min before immersion into liquid nitrogen. Following this procedure 54 and 83% of apices from MD-2 and Puerto Rico varieties respectively survived.

Details

  • Original title: An improved cryopreservation protocol for pineapple apices using encapsulation-vitrification.
  • Record ID : 2005-2268
  • Languages: English
  • Source: CryoLetters - vol. 25 - n. 6
  • Publication date: 2004/11

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