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PRESERVATION OF TAENIA COLI BY FREEZING AND STORAGE AT 77 K (-196 DEG C).

Author(s) : PENNINCKX F.

Type of article: Article

Summary

SOME DAMAGING EFFECTS THAT OCCUR DURING CRYOPRESERVATION BY FREEZING TO 77 K HAVE BEEN EVALUATED IN RABBIT TAENIA COLI BY ANALYZING THE PROPORTIONAL RECOVERY OF ACETYLCHOLINE-AND HISTAMINE-INDUCED MAXIMAL CONTRACTIONS. DMSO WAS USED AS THE CRYOPROTECTANT ; IT REVERSIBLY ABOLISHES SPONTANEOUS CONTRACTILITY EVEN AFTER INCUBATION AT 310 K (37 DEG C) DURING 2 HR. PROGRAMMED FREEZING AT 0.6 K/MIN WITH COMPENSATION FOR THE LATENT HEAT OF FUSION AND WARMING AT 35 K/MIN PROVED TO BE SLIGHTLY SUPERIOR TO PROGRAMMED COOLING WITHOUT COMPENSATION AND SLOWER WARMING. PREVIOUS RESULTS CAN BE IMPROVED BY CHANGING THE FREEZING RATE AND THE COMPOSITION OF THE INCUBATING AND CRYOPROTECTING MEDIUM.

Details

  • Original title: PRESERVATION OF TAENIA COLI BY FREEZING AND STORAGE AT 77 K (-196 DEG C).
  • Record ID : 1987-2447
  • Languages: English
  • Source: Cryobiology - vol. 23 - n. 3
  • Publication date: 1986

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