ShareAN IMPROVED PRESERVATION TECHNIQUE FOR CELLS OF HEMOPOIETIC ORIGIN.
Author(s) : CONSCIENCE J. F., FISCHER F.
Type of article: Article
Summary
A NEW TECHNIQUE USES A COMBINATION OF 10% POLYVINYLPYRROLIDONE OR HYDROXYETHYLSTARCH WITH 15% GLYCEROL, A SLOW STEP-WISE ADDITION OF THE CRYOPROTECTANTS BEFORE FREEZING, COOLING AT 4 K/MIN, RAPID REWARMING, AND A SLOW STEP-WISE DILUTION AFTER THAWING. THIS TECHNIQUE HAS GIVEN IMPROVED RECOVERY RATES WITH A NUMBER OF BASOPHIL/MAST CELL LINES, AS WELL AS A WIDE RANGE OF HEMOPOIETIC CELLS INCLUDING HYBRIDOMAS.
Details
- Original title: AN IMPROVED PRESERVATION TECHNIQUE FOR CELLS OF HEMOPOIETIC ORIGIN.
- Record ID : 1986-2107
- Languages: English
- Source: Cryobiology - vol. 22 - n. 5
- Publication date: 1985
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FROZEN STARTERS FROM INTERNAL PH CONTROL GROWN ...
- Author(s) : THUNELL R. K., SANDINE W. E., BODYFELT F. W.
- Date : 1984/01
- Languages : English
- Source: Egypt. J. Dairy Sci. - vol. 67 - n. 1
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- Author(s) : YUASA S.
- Date : 1982
- Languages : Japanese
- Source: Refrigeration - vol. 57 - n. 656
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NONBENEFICIAL EFFECTS OF GLYCEROL ON THE OOCYTE...
- Author(s) : JEYENDRAN R. S.
- Date : 1985
- Languages : English
- Source: Cryobiology - vol. 22 - n. 5
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EFFECT OF GLYCEROL ON THE VIABILITY OF FOWL SPE...
- Author(s) : LAKE P. E., BUCKLAND R. B., RAVIE O.
- Date : 1980/06
- Languages : English
- Source: Cryo-Letters - vol. 1 - n. 9
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- Author(s) : POLLOCK G. A., et al.
- Date : 1991/10
- Languages : English
- Source: Cryobiology - vol. 28 - n. 5
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