ShareAN IMPROVED PRESERVATION TECHNIQUE FOR CELLS OF HEMOPOIETIC ORIGIN.
Author(s) : CONSCIENCE J. F., FISCHER F.
Type of article: Article
Summary
A NEW TECHNIQUE USES A COMBINATION OF 10% POLYVINYLPYRROLIDONE OR HYDROXYETHYLSTARCH WITH 15% GLYCEROL, A SLOW STEP-WISE ADDITION OF THE CRYOPROTECTANTS BEFORE FREEZING, COOLING AT 4 K/MIN, RAPID REWARMING, AND A SLOW STEP-WISE DILUTION AFTER THAWING. THIS TECHNIQUE HAS GIVEN IMPROVED RECOVERY RATES WITH A NUMBER OF BASOPHIL/MAST CELL LINES, AS WELL AS A WIDE RANGE OF HEMOPOIETIC CELLS INCLUDING HYBRIDOMAS.
Details
- Original title: AN IMPROVED PRESERVATION TECHNIQUE FOR CELLS OF HEMOPOIETIC ORIGIN.
- Record ID : 1986-2107
- Languages: English
- Source: Cryobiology - vol. 22 - n. 5
- Publication date: 1985
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Indexing
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DEEP FREEZE PRESERVATION OF CATTLE EMBRYOS.
- Author(s) : FALGE R.
- Date : 1983
- Languages : German
- Source: Mon.hefte Vet.med. - vol. 38 - n. 22
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CRYOPRESERVATION OF MURINE EMBRYOS WITH TREHALO...
- Author(s) : HONADEL T. E., KILLIAN G. J.
- Date : 1988
- Languages : English
- Source: Cryobiology - vol. 25 - n. 4
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STORAGE OF ERYTHROCYTES AT 253 TO 249 K (-20 TO...
- Author(s) : DAZYNSKI J.
- Date : 1981/06/01
- Languages : English
- Source: Cryosurgery and medical applications of refrigeration: current situation and perspectives.
- Formats : PDF
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FREEZE PRESERVATION OF HUMAN RED CELLS AT 248 K...
- Author(s) : TOURSEL W. D.
- Date : 1981/06/01
- Languages : English
- Source: Cryosurgery and medical applications of refrigeration: current situation and perspectives.
- Formats : PDF
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FROZEN STARTERS FROM INTERNAL PH CONTROL GROWN ...
- Author(s) : THUNELL R. K., SANDINE W. E., BODYFELT F. W.
- Date : 1984/01
- Languages : English
- Source: Egypt. J. Dairy Sci. - vol. 67 - n. 1
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