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Effects of four cryoprotectants in combination with two vehicle solutions on cultured vascular endothelial cells.

Author(s) : POLLOCK G. A., et al.

Type of article: Article

Summary

The first step in organ cryopreservation will be the maintenance of endothelial cell integrity during perfusion of cryoprotective agents. The authors compare the effects of incubation on cultured porcine endothelial cells at 10 deg C for 1 h with the cryoprotective agents glycerol, DMSO, ethanediol, and propane-1,2-diol in the vehicle solutions RPS-2 (high potassium, high glucose) and HP-5NP (low potassium, high sodium), both with and without added colloids. Osmotic stress appeared to be the major problem with glycerols use. Results are given and the different cryoprotective agents are compared.

Details

  • Original title: Effects of four cryoprotectants in combination with two vehicle solutions on cultured vascular endothelial cells.
  • Record ID : 1992-3067
  • Languages: English
  • Source: Cryobiology - vol. 28 - n. 5
  • Publication date: 1991/10

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