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Analysis of cryoprotective agent transport in the cryopreservation of algae.

Author(s) : TANAKA J. Y., DILLER K. R., WALSH J. R., et al.

Summary

Glycerol and dimethyl sulfoxide are by far the most widely used cryoprotective agents (CPA), but studies have shown methanol to be the CPA of choice for algae. Cell membranes are more permeable to methanol than to water. Algal cells respond to osmotic stress by volumetric alterations governed by the combined permeability and elastic properties of the plasma membrane and wall. A series of experiments has been conducted to measure the relative response of Chlorococcum texanum to step-wise exposures of glycerol and methanol. The relative osmotic behaviour of each cell was calibrated by initial mixing with a sucrose solution. Subsequently, a 200 mOsm solution of a CPA was introduced rapidly into the cell environment, causing a swelling back toward the native state. Image analysis demonstrates a much slower period required for equilibration with glycerol than with methanol.

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Details

  • Original title: Analysis of cryoprotective agent transport in the cryopreservation of algae.
  • Record ID : 2000-1619
  • Languages: English
  • Source: 20th International Congress of Refrigeration: Refrigeration into the Third Millennium.
  • Publication date: 1999/09/19

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