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Apex cryopreservation of several strawberry genotypes by two encapsulation-dehydration methods.

Author(s) : CLAVERO RAMÍREZ I., GÁLVEZ FARFÁN J., LÓPEZ ARANDA J. M., et al.

Type of article: Article

Summary

This paper presents results of a study to develop cryopreservation procedures for apices of several strawberry genotypes. Five Fragaria x ananassa Duch. cultivars and two wild species (F. chiloensis and F. virginiana) were screened using the encapsulation-dehydration method and/or a protocol which compromises vitrification and encapsulation-dehydration. Apices were encapsulated in an alginate gel, precultured on media containing high levels of sucrose (0.8 M, conventional protocol), or a combination of 0.4 M sucrose and 2 M glycerol. Recovery rates varied among genotypes (23-63%). The latter method reduced considerably the time needed for the cryogenic procedure by eliminating the pretreatment with 0.8 M sucrose for 19 h prior to dehydration, as required by the conventional procedure.

Details

  • Original title: Apex cryopreservation of several strawberry genotypes by two encapsulation-dehydration methods.
  • Record ID : 2006-0326
  • Languages: English
  • Source: CryoLetters - vol. 26 - n. 1
  • Publication date: 2005/02

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