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Blastomere disintegration of cryopreserved two-cell zona-free mouse embryos.

Author(s) : SANDALINAS M., GROSSMANN M., EGOZCUE J., SANTALO J.

Type of article: Article

Summary

2-cell zona-free embryos were frozen at four different final temperatures (-7, -20, -30, and -196 deg C) either with or without the induction of ice formation. Results show that at least two factors play a role in disaggregation. The absence of zona pellucida allows maximal expansion when dehydration/rehydration occurs, and simultaneously it seems that there is a depressed action of cell-to-cell adhesion proteins (at final temperatures between -20 and -30 deg C). Ice consolidation (at final temperatures lower than -30 deg C) has a mechanical effect that also leads to disaggregation.

Details

  • Original title: Blastomere disintegration of cryopreserved two-cell zona-free mouse embryos.
  • Record ID : 1995-3882
  • Languages: English
  • Source: Cryo-Letters - vol. 15 - n. 6
  • Publication date: 1994/11
  • Document available for consultation in the library of the IIR headquarters only.

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