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Calculation of corneal endothelial cell volume during the addition and removal of cryoprotective compounds.

Author(s) : MADDEN P. W., PEGG D. E.

Type of article: Article

Summary

A method is described to calculate cell volume during exposure to and removal of cryoprotective compounds. The osmolality of the solutions is calculated by summing the values for individual components on a molal basis and applying experimentally-determined or assumed osmotic coefficients. Transient volumes are calculated assuming only osmotically-driven water flux, and equilibrium volumes are calculated assuming complete penetration of low-molecular weight cryoprotectants. The calculations are used to design procedures to reduce osmotic injury during corneal cryopreservation.

Details

  • Original title: Calculation of corneal endothelial cell volume during the addition and removal of cryoprotective compounds.
  • Record ID : 1993-0545
  • Languages: English
  • Source: Cryo-Letters - vol. 13 - n. 1
  • Publication date: 1992/01
  • Document available for consultation in the library of the IIR headquarters only.

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